Evidence Based This post has 123 references
4.6 /5
0

Erythrocyte Sedimentation Rate (ESR) Test & Normal Levels

Written by Joe Cohen, BS | Last updated:
Jonathan Ritter
Medically reviewed by
Jonathan Ritter, PharmD, PhD (Pharmacology) | Written by Joe Cohen, BS | Last updated:
Erythrocytes are red blood cells; The ESR blood test measures their sedimentation rate

The erythrocyte sedimentation rate (ESR) blood test checks for chronic inflammation, but it’s not sensitive. Often called the “sickness index,” doctors can also use it to track the progress of specific diseases. Read on to find out how ESR works, what can impact ESR levels in your body, and how this lab marker relates to your health.

What Is the Erythrocyte Sedimentation Rate (ESR)?

Definition

Erythrocyte sedimentation rate (ESR) was discovered in 1987 [1, 2].

The ESR is the rate at which red blood cells (erythrocytes) sink to the bottom (sedimentation) of a tube in one hour [3, 4].

The main factors affecting ESR are hematocrit (percentage of red blood cells in your blood) and blood proteins, such as fibrinogen [5].

Erythrocyte Sedimentation Rate (ESR) Blood Test

Erythrocyte sedimentation rate (ESR) test or “sed rate test”, is a blood test that checks for inflammation. It measures the distance in millimeters that red blood cells fall in one hour (mm/hr) [6, 7].

There are a few ways that this is measured, such as the Westergren method, Wintrobe Method, MicroESR, and automated methods [8, 9, 10].

Westergren Method

The Westergren method is considered the gold standard in measuring ESR [11].

The blood sample is mixed with sodium citrate (4:1). Then it is mixed into a Westergren-Katz tube (2.5 mm diameter) until the 200 mm mark.

Next, the tube is set vertically, at room temperature (18 – 25 °C), for one hour.

At the end of an hour, they measure how far the red blood cells have settled. This distance is ESR.

In the modified Westergren method, edetic acid is used instead of sodium citrate [8, 9, 10].

Other Methods

The Wintrobe method is less sensitive than the Westergren method and the maximal values may be misleading [12, 13].

The micro ESR method is quick (20 min) and popular for babies, as the test requires very little blood. It is also useful for diagnosing neonatal sepsis [14, 15, 16].

Automated methods are faster, easy to use, and could be better predictors for autoimmune diseases. Yet, their sensitivity to technical procedures (blood mixing, tube sizes, etc.) could influence the test results [17, 18, 19, 20, 21, 22, 23, 24].

ESR is most commonly measured using the Westergren method. Micro ESR can be used in newborns and it requires only a small amount of blood.

When to Get a Sed Rate Test

Your doctor may order an ESR test if you have the following symptoms [25]:

What Can The ESR Test Reveal?

Factors and disease shown here are commonly associated with abnormal ESR values. Work with your doctor or other health care professional for an accurate diagnosis.

1) Inflammation

The ESR test checks if you have an inflammation [3].

In inflammation, certain proteins will appear in the blood, such as fibrinogen. These proteins cause red blood cells to cling together and form clumps. This makes them heavier, so they fall faster, which increases the ESR [5, 26, 3].

Therefore, high ESR shows inflammation. The higher the ESR level, the higher the inflammation [5, 26, 3].

But, the ESR test is not very sensitive (so it can’t pick up all inflammation) nor specific, so it can not diagnose specific diseases [6].

2) Screening for Specific Diseases

The ESR test could help with the diagnosis of certain diseases:

  • Polymyalgia rheumatica (an inflammatory disease which causes muscle pain and stiffness) [27, 28, 29]
  • Giant cell arteritis (inflammation of blood vessels) [30, 31, 32, 33, 34]
  • Cancer [35, 36]
  • Bone infections [37, 38, 39]
  • Subacute thyroiditis (inflammation of the thyroid) [40, 41, 42]
  • Ulcerative colitis [43]

3) Progression of Specific Conditions

The ESR test cannot diagnose diseases, but it can track the progress of specific conditions [44]:

  • Heart disease [45, 46, 47]
  • Cancer [48, 5, 49]
  • Rheumatoid arthritis [50, 51, 52]
  • Systemic lupus erythematosus (SLE) [53, 54]
  • Sickle-cell disease [55, 56, 57]

4) Serious Conditions

ESR levels higher than 100 mm/hr could suggest a serious disease, such as infection, heart disease, or cancer [58, 5, 3, 6].

ESR levels higher than normal may predict cancer or cancer progression, like metastasis [59, 60, 61, 62, 63].

Your doctor may order an ESR test to check for specific inflammatory or chronic conditions, along with other tests. ESR is considered a non-specific, non-sensitive marker of chronic inflammation.

Normal ESR Levels

Age Men Women
Younger than 50 0-15 mm 0-20 mm
Older than 50 0-20 mm 0-30 mm

Children should have an ESR lower than 10mm [3].

A low ESR is normal and does not cause any symptoms [25].

Factors That Increase ESR Levels

Disorders and diseases:

  • Inflammation, infection, or malignant diseases can increase ESR rates [3, 5, 26, 64, 65, 66]
  • Women tend to have higher ESR rates [3, 6, 5, 67]
  • Old age [3, 6, 5, 67, 68]
  • Anemia; reduced hematocrit increases ESR levels [3, 5, 6, 65]
  • Macrocytosis (large red blood cells) [3, 5]
  • Polycythemia (increased production of red blood cells) [3, 5, 69, 8, 70]
  • Increased fibrinogen levels [3, 5]
  • Pregnancy [71, 72]
  • Diabetes [3, 5, 73]
  • Kidney failure [74]
  • Chronic heart failure [75]
  • Obesity [6, 5]
  • Hyperlipidemia (high concentrations of fats in the blood) [76]
  • Heart disease [77, 45, 46]
  • Polymyalgia rheumatica (an inflammatory disorder where there is muscle pain around the shoulders and hips) [78, 28, 79]
  • Subacute thyroiditis [40]
  • Alcoholic liver disease, which can cause decreased albumin production, and thus increase ESR [80]
  • Crohn’s disease and ulcerative colitis [81, 82]
  • Kidney failure [74]
  • Temporal/giant cell arteritis (inflammation in blood cells around the scalp) [83, 84]
  • Multiple myeloma [85]
  • Waldenstrom’s macroglobulinemia (tumors that make large amounts of immunoglobulins) [86, 87]
  • Atherosclerosis (plaque buildup in the arteries) and stroke [88]
  • Cancer risk, progression, and death [35, 89, 90]
Many inflammatory disorders and chronic diseases are associated with high ESR values.

Drugs and supplements:

  • Iodine, when it causes thyroid problems [91]
  • High ginger consumption, when it’s linked to subacute thyroiditis [92]
  • Birth control [93]
  • Smoking [94, 95, 96, 97]
  • Alcohol [80, 98]
  • Dextran [99]

Technical errors during the test such as a tilted tube or dilution error can also give a false high ESR result [3].

Factors That Decrease ESR Levels

When red blood cells are smaller, they will drop slower, hence a lower ESR.

  • Red blood cells diseases: extreme leukocytosis, polycythemia, microcytosis, sickle cell disease, spherocytosis, acanthocytosis, and anisocytosis [3, 5, 69, 8, 55]
  • Protein abnormalities: hypofibrinogenemia, hypogammaglobulinemia, and dysproteinemia with hyperviscosity state [3, 5, 8, 65, 100, 70]
  • NSAIDs, cortisone, anesthetic drugs, levamisole, and prednisone [101, 5, 8, 102, 84]
Low ESR is usually normal, but some factors that excessively lower red blood cells have also been linked to low ESR values.

ESR Genetics

Genes That Affect ESR

These SNPs/genes are associated with a higher ESR:

  • rs3750996 (G/G) [103], rs3750996 (G-C) [103]
  • rs2066865 (C>T) (minor allele) [104]
  • rs3750994 (G-C) is related to higher ESR levels [103]

High fibrinogen levels make the red blood cells heavier and increase the ESR. So, genes increasing fibrinogen production could theoretically increase ESR levels. These include:

These SNPs/genes are associated with lower ESR levels:

  • rs630337 (T/C) [113]
  • rs11117956 (T/G) [113]
  • rs11549407 (A/G) [113]
  • rs650877 (G/A) [114]
  • rs11118131 (T/C) [114]
  • rs677066 (G/A) [114]
  • rs6691117 (G/A) [114]
  • rs12034383 (G/A) [114]
  • rs1043879 [114]
  • rs3091242 [114]
  • rs873308 [114]
  • rs10903129 [114]
  • rs7527798 [114]

Low fibrinogen levels make the red blood cells lighter and decrease the ESR. So, genes decreasing fibrinogen production may theoretically reduce ESR levels:

  • rs1800787 (C>T) is associated with low fibrinogen, slow initiation of the coagulation cascade, and possibly childhood pneumonia [106, 115].
  • rs148685782 (G>C) is associated with low blood fibrinogen levels and hypofibrinogenemia [116, 117].

ESR and C-Reactive Protein (CRP)

In inflammation, the liver is producing a protein called C-reactive protein (CRP). The CRP blood test checks whether you have inflammation or infection. CRP levels higher than 10 mg/dL show infection [118, 119, 120].

CRP test is used most times together with the ESR test [121, 122, 123].

C-reactive protein is more sensitive than ESR and produces less false negative/false positive results than ESR [25].

CRP is better for checking and tracking the progress of acute inflammations, and infections [25, 121, 122].

ESR is better for checking and tracking the progress of chronic inflammations, and infections [25, 121, 122].

Both ESR and CRP can increase with inflammation, but CRP may be a better marker of acute and ESR of chronic inflammation and infection.

Limitations and Caveats

While there are many studies examining ESR, almost all of them are population-based, which means they can associate high or low ESR values with certain conditions but not establish them as their cause. Additional limitations of some of these studies include being based on older data or only conducted with men.

More clinical trials investigating ESR should be undertaken.

Takeaway

Erythrocyte sedimentation rate (ESR) test or “sed rate test” is a blood test that checks for inflammation.

It is most commonly measured using the Westergren method, the gold standard in assessing ESR. Micro ESR is another method that can be used in newborns that requires only a small amount of blood.

Your doctor may order an ESR test if you show signs or symptoms of infection or inflammation, such as fever, headache, and pain. They may also order this test to screen for specific diseases or to monitor chronic autoimmune diseases like lupus and rheumatoid arthritis.

The normal ESR range should be below 15 or 30 mm, depending on gender and age.

Read Next

About the Author

Joe Cohen, BS

Joe Cohen, BS

Joe Cohen won the genetic lottery of bad genes. As a kid, he suffered from inflammation, brain fog, fatigue, digestive problems, anxiety, depression, and other issues that were poorly understood in both conventional and alternative medicine. Frustrated by the lack of good information and tools, Joe decided to embark on a journey of self-experimentation and self-learning to improve his health--something that has since become known as “biohacking”. With thousands of experiments and pubmed articles under his belt, Joe founded SelfHacked, the resource that was missing when he needed it. SelfHacked now gets millions of monthly readers. Joe is a thriving entrepreneur, author and speaker. He is the CEO of SelfHacked, SelfDecode and LabTestAnalyzer. His mission is to help people gain access to the most up-to-date, unbiased, and science-based ways to optimize their health.
Joe has been studying health sciences for 17 years and has read over 30,000 PubMed articles. He's given consultations to over 1000 people who have sought his health advice. After completing the pre-med requirements at university, he founded SelfHacked because he wanted to make a big impact in improving global health. He's written hundreds of science posts, multiple books on improving health, and speaks at various health conferences. He's keen on building a brain-trust of top scientists who will improve the level of accuracy of health content on the web. He's also founded SelfDecode and LabTestAnalyzer, popular genetic and lab software tools to improve health.

RATE THIS ARTICLE

1 Star2 Stars3 Stars4 Stars5 Stars
(5 votes, average: 4.60 out of 5)
Loading...

FDA Compliance

The information on this website has not been evaluated by the Food & Drug Administration or any other medical body. We do not aim to diagnose, treat, cure or prevent any illness or disease. Information is shared for educational purposes only. You must consult your doctor before acting on any content on this website, especially if you are pregnant, nursing, taking medication, or have a medical condition.

Leave a Reply

Your email address will not be published. Required fields are marked *

Related Articles View All

caret-downclock-grayclosecomment-bubbledown-anglefacebook-squarehamburgerinstagram-squarelinkedin-squareminuspauseplayplustwitter-squareup-angle